|
| Status |
Public on Aug 14, 2023 |
| Title |
CD11b-Ly6G- mouse 1 |
| Sample type |
RNA |
| |
|
| Source name |
Cecum infected with C. difficile strain VPI 10463 (ATCC 43255)
|
| Organism |
Mus musculus |
| Characteristics |
cell type: non-myeloid cells
|
| Treatment protocol |
Cells were digested with Liberase, FACS was performed using FACSAria III and collected in PBS + 2% FBS.
|
| Growth protocol |
After C. difficile intection for 2 days, murine ceca were biopsied.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA extracted using the RNEasy Microkit
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Libraries were prepared using the NuGen WT-pico kit and hybridized on Affymetrix Mouse Gene ST 2.1
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G
|
| Description |
Gene expression data from gastric non-myeloid cells after H. felis infection
|
| Data processing |
Gene expression values were calculated using robust multi-array average (RMA) (RMA) I
|
| |
|
| Submission date |
Aug 11, 2023 |
| Last update date |
Aug 14, 2023 |
| Contact name |
Bioinformatics Core |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Street address |
BRCF Bioinformatics Core, 2800 Plymouth Rd
|
| City |
Ann Arbor |
| State/province |
Mi |
| ZIP/Postal code |
48109-2800 |
| Country |
USA |
| |
|
| Platform ID |
GPL17400 |
| Series (2) |
| GSE240718 |
Gene expression in gut CD11b+ Ly6G+ myeloid-derived suppressor cells (MDSCs), which arise during long-term chronic infection. |
| GSE240720 |
The Cxcr2+ subset of the S100A8+ Gastric Granylocytic Myeloid-Derived Suppressor Cell Population Regulates Gastric Pathology |
|
