|
| Status |
Public on Nov 17, 2024 |
| Title |
Control 5 |
| Sample type |
SRA |
| |
|
| Source name |
Left ventricle
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Left ventricle
genotype: C56Bl6
treatment: control diet for 6 weeks
|
| Treatment protocol |
C56Bl6 fed with a standard diet (control group) or High-Fat + L-NAME (HFpEF group) for 6 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Left ventricular samples were harvested in ice-cold PBS, flash frozen on LN2, and RNA extracted using RNeasy kit (QIAzol, Qiagen).
Total RNA-Seq library construction was performed using the Swift Biosciences RNA Library Kit (Swift Biosciences, Ann Arbor, MI), following rRNA depletion using Lexogen RiboCop Depletion Kit Human/Mouse/Rat v2 (Lexogen Inc., Greenland, NH). Final library concentration was measured via the Qubit 1X dsDNA HS Assay kit (ThermoFisher Scientific) and library size was evaluated on the 4200 TapeStation using the Agilent D1000 ScreenTape System (Agilent Technologies).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
LVCtr5
|
| Data processing |
The reads were first mapped to the latest UCSC transcript set using Bowtie2 version 2.1.0 and the gene expression level was estimated using RSEM v1.2.15.
Assembly: mm10
Supplementary files format and content: tab-delimited text files include raw count values for each Sample
|
| |
|
| Submission date |
Aug 08, 2023 |
| Last update date |
Nov 17, 2024 |
| Contact name |
Thassio Mesquita |
| E-mail(s) |
[email protected]
|
| Organization name |
Cedars-Sinai Medical Center
|
| Department |
Smidt Heart Institute
|
| Street address |
8700 Beverly Boulevard
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90048 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE240400 |
Upregulated FoxO1 promotes arrhythmogenesis in mice with heart failure and preserved ejection fraction [6 wks] |
| GSE240404 |
Upregulated FoxO1 promotes arrhythmogenesis in mice with heart failure and preserved ejection fraction |
|
| Relations |
| BioSample |
SAMN36893097 |
| SRA |
SRX21302023 |
