|
| Status |
Public on Aug 02, 2012 |
| Title |
SKOV3, IFN, 6h, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
SKOV3 cells, IFN treated 6h
|
| Organism |
Homo sapiens |
| Characteristics |
time: 6h
treatment: IFN
cell line: SKOV3 human ovarian adenocarcinoma cell line
|
| Treatment protocol |
Confluent cells were treated with interferon-alpha (500 U/ml), U0126 (20uM) or both for 6 or 12 hours.
|
| Growth protocol |
Cells were plated 6-well plates the day prior to treatment.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TriZOL (Invitrogen) following the manufacturer's protocol. RNA was treated with Turbo DNA-free to remove genomic DNA contamination.
|
| Label |
biotin
|
| Label protocol |
500 ng of RNA was used for labeling using the Ambion WT kit. Labeling was performed by The Centre for Applied Genomics (Toronto, Canada) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
Hybridization was performed by The Centre for Applied Genomics (Toronto, Canada) according to the manufacturer's protocol (FS450_0007).
|
| Scan protocol |
Scanning was performed with the Affymetrix GeneChip Scanner 3000 by The Centre for Applied Genomics (Toronto, Canada) according to the manufacturer's protocol.
|
| Description |
gene expression data from SKOV3 cells treated with Inteferon-alpha for 6 hours
|
| Data processing |
Data were analyzed using Bioconducter 2.6. Pre-processing was performed with the Oligo package using rma normalization. Each cell type was analyzed independently, relative to the time-matched control.
|
| |
|
| Submission date |
Jul 28, 2011 |
| Last update date |
Aug 02, 2012 |
| Contact name |
Sherri L Christian |
| E-mail(s) |
[email protected]
|
| Phone |
(709)864-8550
|
| Organization name |
Memorial University of Newfoundland
|
| Department |
Biochemistry
|
| Street address |
232 Elizabeth Ave
|
| City |
St. John's |
| State/province |
Newfoundland |
| ZIP/Postal code |
A1B3X9 |
| Country |
Canada |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE31019 |
Suppression of IFN-induced transcription underlies IFN defects generated by activated Ras/MEK in human cancer cells |
|
