|
| Status |
Public on Aug 23, 2011 |
| Title |
Secondary_Chronic, rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Splenic secondary effector CD8 T cells, day 8 post-chronic infection
|
| Organism |
Mus musculus |
| Characteristics |
strain: P14 TCR transgenic
tissue: spleen
cell type: CD8 T cells
cd8 t cell effector type: secondary
lcmv infection type: chronic
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Spleen cells were isolated and sorted using a BD Biosciences Aria cell sorter. RNA was isolated using the Qiagen RNeasy kit according to the standard manufacturer's protocol including the optional on-column DNase treatment.
|
| Label |
biotin
|
| Label protocol |
1st and 2nd strand synthesis, followed by Spia amplification using the Nugen WT pico kit to generate cDNA. Fragmentation and biotinylation using Nugen cat# 4200 following standard Nugen/Affymetrix protocols.
|
| |
|
| Hybridization protocol |
5 ug was hybridized following standard Nugen/Affymetrix protocols.
|
| Scan protocol |
Scanned with Affymetrix using AGCC v. 1.1.
|
| Data processing |
Analyzed on Affymetrix Expression Console v. 1.1 using a GC-RMA normalization algorithm producing log base 2 results.
|
| |
|
| Submission date |
Jul 26, 2011 |
| Last update date |
Aug 23, 2011 |
| Contact name |
Erin West |
| E-mail(s) |
[email protected]
|
| Organization name |
Emory University
|
| Street address |
1510 Clifton Rd NE RRC room G211
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE30962 |
Primary and secondary CD8 T cells during acute and chronic LCMV infection |
|
