|
| Status |
Public on Jul 09, 2012 |
| Title |
2527_6511_31084_928-1 |
| Sample type |
RNA |
| |
|
| Source name |
Mammary Tumor
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
tissue type: Mammary Tumor
histology: T58A Low Expressing, EMT
|
| Treatment protocol |
NA
|
| Growth protocol |
Animal use and husbandry was in accordance with institutional and federal guidelines.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA from mouse mammary tumors was extracted using the Qiagen RNeasy Midi kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
The resulting biotin-labeled RNA samples were then hybridized with Affymetrix Mouse 430A 2.0 GeneChip arrays according to the manufacturer's instructions (Affymetrix, Santa Clara,
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from mouse mammary tumor tissue.
|
| Data processing |
The data were analyzed using Stratagene's ArrayAssist Enterprise using Affymetrix default analysis settings.
|
| |
|
| Submission date |
Jul 20, 2011 |
| Last update date |
Jul 09, 2012 |
| Contact name |
Daniel Patrick Hollern |
| Phone |
(517)884-5042
|
| Organization name |
Michigan State University
|
| Street address |
2194 Biomedical Physical Sciences
|
| City |
East Lansing |
| State/province |
MI |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE30805 |
Expression data from low expressing T58A Mutant Myc Induced Tumors |
|
