|
| Status |
Public on Jul 09, 2012 |
| Title |
BMDC, wt, replicate 3 |
| Sample type |
RNA |
| |
|
| Source name |
BMDC, wt
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
genotype/variation: wild type
cell type: bone marrow derived dendritic cells
|
| Treatment protocol |
Bone marrow cells were cultured in RPMI growth media containing 10% Fetal bovine serum and in the presence of GM-CSF (20 ng/ml). Cell culture medium was replenished with fresh medium every two days. On day 7 cells were used for isolation of RNA using the Trizol method.
|
| Growth protocol |
Bone marrow cells were isolated from the femurs of mice and in vitro cultured for seven days. Cell were induced to differentiate into BMDCs using 20ng/ml of GM-CSF for seven days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Trizol method (Invitrogen)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA was prepared according to the Affymetrix 3' IVT Express protocol starting with 100 ng of total RNA (GeneChip 3’ IVT Express Kit 702646 Rev. 8, 2008-2010, Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 micrograms of cRNA was hybridized for 16 hr at 45C on each array. GeneChips were washed and stained in the Affymetrix GeneTitan system.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneTitan system.
|
| Data processing |
Probe level normalization and signal summarization was performed using the Robust Multi-chip Algorithm (RMA) as implemented in the Affymetrix Expression Console software version 1.1
|
| |
|
| Submission date |
Jul 19, 2011 |
| Last update date |
Jul 10, 2012 |
| Contact name |
Geoffrey Neale |
| E-mail(s) |
[email protected]
|
| Organization name |
St Jude Childrens Research Hospital
|
| Department |
Hartwell Center
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE30769 |
Comparison of gene expression profiles in naïve WT and Asc-null bone marrow derived dendritic cells |
|
