|
| Status |
Public on Jul 28, 2011 |
| Title |
infected primary RPE, biological replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
hRPE cells 24h post-infection with WNV
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: hRPE
disease state: 24h post-infection with WNV
gender: male
|
| Treatment protocol |
Cells were infected with 1 plaque forming unit per cell of West Nile Virus
|
| Growth protocol |
Primary cells were extracted from cadaveric eyes and ARPE19 cells were obtained from the ATCC. Cells were then grown at 30ºC with 5% CO2 in DMEM + 10%FCS
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After 24h post infection, cells were lysed with Trizol and RNA was extracted using Rneasy kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 1ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, cRNA was hybridized onto Human Exon 1.0 ST chips for 16h. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned with the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene expression data from infected cells
|
| Data processing |
Data was analysed with the GeneSping Gx11 program using RMA function
|
| |
|
| Submission date |
Jul 15, 2011 |
| Last update date |
Jul 28, 2011 |
| Contact name |
Luis Munoz-Erazo |
| E-mail(s) |
[email protected]
|
| Phone |
+61293516157
|
| Organization name |
University of Sydney
|
| Department |
Department of Pathology
|
| Lab |
Viral Immunopathology Unit
|
| Street address |
Room 260, Blackburn building D06
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2006 |
| Country |
Australia |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE30719 |
Microarray Analysis of West Nile Virus infected Human Retinal Pigment Epithelium |
|
