|
| Status |
Public on Jul 17, 2023 |
| Title |
gastrocnemius muscle, isolated from wildtype mouse, replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
gastrocnemius muscle, isolated from 8-wk old female wildtype (C57BL/6J) mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wildtype (C57BL/6J)
age of mice (wks): 8
Sex: female
|
| Treatment protocol |
Gastrocnemius muscles were harvested from 8-week-old female control (WT) and muscle-specific Sel1L deficient (KO) Mice.
|
| Growth protocol |
Muscle-specific SEL1L (Sel1LMLC) deficient mice were generated by crossing Sel1Lf/f mice with mice expressing Cre driven by the myosin light chain-promoter (MLC), mice that were Cre-negative were used as controls. All mice strains used were on C57BL/6J background and were fed a regular chow diet (13% fat, 57% carbohydrate, and 30% protein; LabDiet 5L0D) with free access to water.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using RNeasy micro kit (Qiagen) including a column for elimination of genomic DNA as per manufacturer’s instructions. RNA concentration was determined using NanoDrop 2000 UV-Vis Spectrophotometer. The quality and concentration were determined using the RNA 6000 Nano kit on an Agilent 2100 bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Total RNA (100ng per sample) was labeled with the Whole-Transcript Sense Target Assay (Affymetrix, Santa Clara, CA, USA; P/N 900652).
|
| |
|
| Hybridization protocol |
Hybridization and washing of the Affymetrix GeneChip Mouse Gene 2.1 ST peg arrays were performed on a GeneTitan Instrument according to the manufacturer's recommendations (Affymetrix, Santa Clara, CA, USA).
|
| Scan protocol |
Arrays were scanned on an Affymetrix GeneTitan instrument (Affymetrix, Santa Clara, CA, USA).
|
| Data processing |
Expression estimates were calculated applying the RMA algorithm in the Bioconductor library 'Oligo' (v1.54.1).
|
| |
|
| Submission date |
Jul 12, 2023 |
| Last update date |
Jul 17, 2023 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
[email protected]
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE237194 |
ER-associated degradation maintains postnatal muscle hypertophy and systemic energy metabolism |
|
