|
| Status |
Public on Jul 11, 2011 |
| Title |
SIRT3KO, high-fat diet, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse liver tissue
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129Sv
tissue: liver
genotype/variation: SIRT3KO
age: 12 weeks
nutrient state: 24h fasted
diet: High-fat diet
|
| Treatment protocol |
Mice were fasted 24 hours, sacrificed, and whole livers were removed.
|
| Growth protocol |
Mice were fed a standard diet, or weaned onto a high-fat Western diet (42% fat)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip Whole Transcript protocol
|
| |
|
| Hybridization protocol |
Affymetrix GeneChip Whole Transcript protocol
|
| Scan protocol |
GCS3000
|
| Description |
Gene expression data from 12 week old SIRT3KO mouse liver tissue fed a high-fat diet
11-KO2
|
| Data processing |
RMA (Robust Multi-array Analysis) was used for normalizing and summarizing probe-level intensity measurements from Affymetrix Gene Chips (Affymetrix Expression Console). Starting with the probe-level data from a set of Gene Chips, the perfect-match (PM) values were background-corrected, normalized and finally summarized resulting in a set of expression measures.
|
| |
|
| Submission date |
Jul 11, 2011 |
| Last update date |
Jul 11, 2011 |
| Contact name |
Matthew Hirschey |
| E-mail(s) |
[email protected]
|
| Organization name |
Duke University Medical Center
|
| Department |
Department of Medicine
|
| Lab |
Duke Molecular Physiology Institute
|
| Street address |
300 N. Duke Street
|
| City |
Durham |
| State/province |
NC |
| ZIP/Postal code |
27701 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE30552 |
Expression data from mice lacking SIRT3 under dietary stress |
|
