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Sample GSM75268 Query DataSets for GSM75268
Status Public on Jul 12, 2006
Title EGF 4h replicate 3
Sample type RNA
 
Source name optic nerve head astrocyte
Organism Rattus norvegicus
Characteristics primary cell culture
Extracted molecule total RNA
Extraction protocol Primary astrocyte cultures from optic nerve head. Cells were lysed in separate groups in RLT buffer (Qiagen).Total RNA was extracted from the tissue lysate using an RNeasy kit (Qiagen).
Label biotin
Label protocol Approximately 3 µg of total RNA was processed to produce biotinylated cRNA targets.
 
Hybridization protocol standard Affymetrix procedures
Scan protocol standard Affymetrix procedures
Description control astrocytes
Data processing Affymetrix Microarray Suite version 5.0
 
Submission date Sep 20, 2005
Last update date Jul 11, 2006
Contact name Bin Liu
E-mail(s) [email protected]
Phone 314-747-4183
Fax 314-747-4211
Organization name Washington University
Department Ophthalmology
Lab Neufeld
Street address 660 S. Euclid
City St. Louis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL1355
Series (1)
GSE5282 Astrocytes in response to activation of EGFR

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
ID_REF VALUE ABS_CALL
1367452_at 29900.6 P
1367453_at 13697.1 P
1367454_at 14364.1 P
1367455_at 21329.9 P
1367456_at 32294.8 P
1367457_at 6680.4 P
1367458_at 2531 P
1367459_at 47578.2 P
1367460_at 34468.2 P
1367461_at 13078.3 P
1367462_at 9503.9 P
1367463_at 14750.8 P
1367464_at 4886.8 P
1367465_at 10023.2 P
1367466_at 8343.6 P
1367467_at 9635.2 P
1367468_at 3463 P
1367469_at 52483.2 P
1367470_at 21464.6 P
1367471_at 5404.8 P

Total number of rows: 31099

Table truncated, full table size 577 Kbytes.




Supplementary data files not provided
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