|
| Status |
Public on Sep 21, 2005 |
| Title |
F2-ob/ob(B) 455 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
(C57BL/6J X BTBR) F2-ob/ob (leptin deficient) mice, Liver, 14 weeks, Gender = M, 4-hr fasting, MOE430A ArrayID = 455A100603, MOE430B ArrayID = 455B100803.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Liver total RNA was extracted from frozen tissue samples with RNAzol reagent (Tel-Test, Inc.). Crude RNA samples were purified with RNeasy mini columns (Qiagen, Inc.) before being subjected to microarray studies. The RNA samples were processed according to the Affymetrix Expression Analysis Technical Manual. A total of 60 MOE430A and MOE430B arrays were used. Quantitative expression levels of all the transcripts were estimated using the RMA algorithm for normalization.
|
| Label |
biotin.
|
| Label protocol |
Approximately 10 µg of total RNA was processed to produce biotinylated cRNA targets according to Affymetrix Expression Analysis Technical Manual.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
(C57BL/6J X BTBR) F2-455. We selected 60 (C57BL/6J X BTBR) F2-ob/ob mice based on the selective phenotyping algorithm (Jin et al. 2004). The 60 mice used in this study were a subset of F2 mice segregating for phenotypes associated with obesity and diabetes (Stoehr et al. 2000). The framework map consists of 145 microsatellite markers spanning the 19 mouse autosomes, with average spacing of 20 cM, augmented by markers in identified regions.
|
| Data processing |
The data were processed with MAS5.0 to generate cell intensity files. Quantitative expression levels of all the transcripts were estimated using the RMA algorithm for normalization (Irizarry et al. 2003).
|
| |
|
| Submission date |
Sep 19, 2005 |
| Last update date |
Sep 20, 2005 |
| Contact name |
Alan D Attie |
| E-mail(s) |
[email protected]
|
| Phone |
608-262-4705
|
| Fax |
608-263-9609
|
| URL |
http://www.biochem.wisc.edu/attie/
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Biochemistry
|
| Lab |
Attie
|
| Street address |
433 Babcock Drive
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL340 |
| Series (1) |
| GSE3330 |
Combined Expression Trait Correlations and Expression Quantitative Trait Locus Mapping |
|
