hypothalami were dissected from one FVB/NJ mouse under a dissection microscope. The samples were snap frozen and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
Label
biotin
Label protocol
10 µg of total RNA was processed to produce biotinylated cRNA targets.
Lockhart-Barlow expression analysis algorithm-calculated signal intensity
ABS CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or reverse present (RP)
DETECTION P-VALUE_LB
'detection p-value', negative log of the p-value from the Wilcoxon signed rank test, relative version that indicates the significance level of the detection call
