hypothalami were dissected from two 129S6/SvEvTac mice under a dissection microscope. The samples were snap frozen and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
Label
biotin
Label protocol
10 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Wild type 8 wk old male 129S6/SvEvTac mouse hypothalamus
Lockhart-Barlow expression analysis algorithm-calculated signal intensity
ABS CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or reverse present (RP)
DETECTION P-VALUE_LB
'detection p-value', negative log of the p-value from the Wilcoxon signed rank test, relative version that indicates the significance level of the detection call
