|
| Status |
Public on Oct 23, 2005 |
| Title |
FVB/NJ cingulate cortex rep1 |
| Sample type |
RNA |
| |
|
| Source name |
cingulate cortex
|
| Organism |
Mus musculus |
| Characteristics |
Wild type 8 wk old male
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cingulate cortices were dissected from two FVB/NJ mice under a dissection microscope. The samples were frozen in the RNALater buffer and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
50 ng of total RNA was processed to produce biotinylated cRNA targets using two rounds of cDNA synthesis and in vitro transcription.
|
| |
|
| Hybridization protocol |
standard Affymetrix procedures
|
| Scan protocol |
standard Affymetrix procedures
|
| Description |
Wild type 8 wk old male FVB/NJ mouse cingulate cortex
|
| Data processing |
Lockhart-Barlow expression analysis algorithm
|
| |
|
| Submission date |
Sep 19, 2005 |
| Last update date |
Feb 27, 2007 |
| Contact name |
Carrolee Barlow |
| Organization name |
The Salk Institute for Biological Studies
|
| Street address |
10010 North Torrey Pines Road
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL81 |
| Series (1) |
| GSE3327 |
Adult mouse gene expression |
|
