|
| Status |
Public on Dec 13, 2011 |
| Title |
R11_785_Sup7 |
| Sample type |
RNA |
| |
|
| Source name |
Tumor cells and centrifuged platelet releasate co-culture
|
| Organism |
Mus musculus |
| Characteristics |
background strain: C57BL/6
cell line: Ep5 (EpRas) cells
platelet description: Wild-type C57BL/6 platelets releasate
treatment protocol: Thrombin treated platelets were centrifuged, releasate was added to EP5 culture for 24 hours followed by RNA isolation
|
| Growth protocol |
Ep5 cells cultured in DMEM 10% FCS, 1% Penicillin/Streptomycin and 2 mM L-Glutamine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen RNAeasy
|
| Label |
biotin
|
| Label protocol |
Nugen Applause® WT-Amp Plus ST System
|
| |
|
| Hybridization protocol |
Performed according to manufacturer's instructions (Affymetrix) for Affymetrix Mouse Gene 1.0 ST arrays.
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G; GeneChip® Command Console.
|
| Data processing |
Data processing was done using Partek Genomics Suite 6.5 software. RMA was used to summarize and quantile normalize and the probe data into probe set expression values using the adjust for GC content and background correction options.
|
| |
|
| Submission date |
Jun 27, 2011 |
| Last update date |
Dec 13, 2011 |
| Contact name |
Charles Arthur Whittaker |
| E-mail(s) |
[email protected]
|
| Organization name |
Koch Institute
|
| Street address |
77 Mass Ave 76-189
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02152 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE27456 |
Direct Signaling Between Platelets and Cancer Cells Induces an Epithelial-Mesenchymal-Like Transition and Promotes Metastasis |
|
