Amygdalae were dissected from two FVB/NJ mice under a dissection microscope. The samples were frozen in the RNALater buffer and stored at -80oC. Total RNA was extracted from the tissue using Trizol reagent (Invitrogen).
Label
biotin
Label protocol
50 ng of total RNA was processed to produce biotinylated cRNA targets using two rounds of cDNA synthesis and in vitro transcription.
Lockhart-Barlow expression analysis algorithm-calculated signal intensity
ABS CALL
the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or reverse present (RP)
DETECTION P-VALUE_LB
'detection p-value', negative log of the p-value from the Wilcoxon signed rank test, relative version that indicates the significance level of the detection call
