|
| Status |
Public on Jul 19, 2011 |
| Title |
normal 2009 150 CD34+ |
| Sample type |
RNA |
| |
|
| Source name |
normal
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: CD34+ mononuclear
disease state: normal
|
| Treatment protocol |
Cell were directly sorted after thawing
|
| Growth protocol |
Mononuclear cells from AML patients (n=46) were thawed, sorted into CD34+ and CD34- subfractions and genome-wide expression analysis was performed using Illumina BeadChip Arrays (HT12 v3). AML CD34+ and CD34- gene expression was compared to a large group of normal CD34+ bone marrow cells (n=31).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard Illumina protocol
|
| Scan protocol |
Illumina i-Scan reader
|
| Description |
2009 150 CD34+
4811636114_H
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Jun 16, 2011 |
| Last update date |
Jul 19, 2011 |
| Contact name |
JJ Schuringa |
| E-mail(s) |
[email protected]
|
| Organization name |
UMCG
|
| Department |
Experimental Hematology
|
| Street address |
Hanzeplein 1
|
| City |
Groningen |
| ZIP/Postal code |
9700RB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6947 |
| Series (1) |
| GSE30029 |
Gene expression profiling in the leukemic stem cell-enriched CD34+ fraction identifies target genes that predict prognosis in normal karyotype AML |
|
