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Sample GSM7426625 Query DataSets for GSM7426625
Status Public on Aug 08, 2024
Title EpCAM POS plastic 06
Sample type RNA
 
Source name plastic epithelial cancer cells from MD/PD-SCCs
Organism Mus musculus
Characteristics tumor type of origin: MD/PD-SCC
cell type: FACS-sorted plastic epithelial cancer cells
Extracted molecule total RNA
Extraction protocol RNA was isolated using magnetic beads RNA Clean XP (Beckman Coulter; catalog # A63987) from a cells lysate.
Label Biotin
Label protocol 10 ng total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and subsequently labeled using (GeneChip Mapping 250K Nsp Assay Kit, Affymetrix catalog # 900766), according to manufacturer's instructions.
 
Hybridization protocol 8 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a GeneAtlas Fluidics Station (Affymetrix, Santa Clara, CA)
Scan protocol Affymetrix MG-430 PM Array were scanned in a GeneAtlas Imaging Station (Affymetrix, Santa Clara, CA)
Description plastic epithelial cancer cells biological replicate 3
Data processing RMA normalization using the normqc function of Bioconductor's MDA package
 
Submission date May 24, 2023
Last update date Aug 08, 2024
Contact name Victoria da Silva-Diz
E-mail(s) [email protected]
Organization name Rutgers University
Street address 195 Little Albany Street
City New Brunswick
ZIP/Postal code 08901
Country USA
 
Platform ID GPL11180
Series (1)
GSE233382 Integrin αV is a prognostic marker for cutaneous squamous cell carcinoma relapse

Data table header descriptions
ID_REF
VALUE RMA normalized values

Data table
ID_REF VALUE
1415670_PM_at 9.83117
1415671_PM_at 10.6776
1415672_PM_at 9.05283
1415673_PM_at 8.42203
1415674_PM_a_at 7.35454
1415675_PM_at 7.95092
1415676_PM_a_at 9.40813
1415677_PM_at 9.88955
1415678_PM_at 9.46189
1415679_PM_at 10.3004
1415680_PM_at 7.94766
1415681_PM_at 8.65502
1415682_PM_at 6.7505
1415683_PM_at 8.80244
1415684_PM_at 4.79564
1415685_PM_at 5.70046
1415686_PM_at 9.27139
1415687_PM_a_at 9.45709
1415688_PM_at 7.08691
1415689_PM_s_at 5.52945

Total number of rows: 45141

Table truncated, full table size 983 Kbytes.




Supplementary file Size Download File type/resource
GSM7426625_6_2017.ga.cel.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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