|
| Status |
Public on Aug 08, 2024 |
| Title |
EpCAM POS 01 |
| Sample type |
RNA |
| |
|
| Source name |
epithelial cancer cells from WD-SCC
|
| Organism |
Mus musculus |
| Characteristics |
tumor type of origin: WD-SCC
cell type: FACS-sorted epithelial cancer cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using magnetic beads RNA Clean XP (Beckman Coulter; catalog # A63987) from a cells lysate.
|
| Label |
Biotin
|
| Label protocol |
10 ng total RNA was amplified using the TransPlex® Complete Whole Transcriptome Amplification Kit (Sigma; reference WTA2) and subsequently labeled using (GeneChip Mapping 250K Nsp Assay Kit, Affymetrix catalog # 900766), according to manufacturer's instructions.
|
| |
|
| Hybridization protocol |
8 µg of cDNA was hybridized per microarray for 16 hours of hybridization at 45ºC, washing and staining of microarrays was performed using a GeneAtlas Fluidics Station (Affymetrix, Santa Clara, CA)
|
| Scan protocol |
Affymetrix MG-430 PM Array were scanned in a GeneAtlas Imaging Station (Affymetrix, Santa Clara, CA)
|
| Description |
full epithelial cancer cells biological replicate 1
|
| Data processing |
RMA normalization using the normqc function of Bioconductor's MDA package
|
| |
|
| Submission date |
May 24, 2023 |
| Last update date |
Aug 08, 2024 |
| Contact name |
Victoria da Silva-Diz |
| E-mail(s) |
[email protected]
|
| Organization name |
Rutgers University
|
| Street address |
195 Little Albany Street
|
| City |
New Brunswick |
| ZIP/Postal code |
08901 |
| Country |
USA |
| |
|
| Platform ID |
GPL11180 |
| Series (1) |
| GSE233382 |
Integrin αV is a prognostic marker for cutaneous squamous cell carcinoma relapse |
|
