|
| Status |
Public on Jun 06, 2011 |
| Title |
Negative Control ASO Treatment rep1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse brain
|
| Organism |
Mus musculus |
| Characteristics |
strain: BACHD
age: 5-months-old
tissue: brain
treatment: Negative Control antisense oligonucleotide (ASO)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy mini kit (cat # 74106, Qiagen) was used to purify total RNA from animal tissues. Tissue was lysed and homogenized, precipitated with ethanol, washed, and then eluted in double distilled RNase free water.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
D1
Negative Control ASO replicate1
|
| Data processing |
The data were normalised using quantile normalisation with lumi in R
|
| |
|
| Submission date |
Jun 06, 2011 |
| Last update date |
Jun 06, 2011 |
| Contact name |
Mahyar Sabripour |
| E-mail(s) |
[email protected]
|
| Organization name |
Isis Pharmaceuticals
|
| Street address |
1896 Rutherford Ave.
|
| City |
Carlsbad |
| State/province |
CA |
| ZIP/Postal code |
92008 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE29751 |
Genomic Analysis of wig-1 Pathways |
|
