RNeasy mini kit (cat # 74106, Qiagen) was used to purify total RNA from animal tissues. Tissue was lysed and homogenized, precipitated with ethanol, washed, and then eluted in double distilled RNase free water.
Label
biotin
Label protocol
Biotinylated cRNA were prepared with the Ambion TotalPrep kit for Illumina arrays
Hybridization protocol
Standard Illumina hybridization protocol
Scan protocol
Standard Illumina scanning protocol
Description
B1 Wig1 ASO replicate1
Data processing
The data were normalised using quantile normalisation with lumi in R