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| Status |
Public on Aug 26, 2024 |
| Title |
c532_drosophila |
| Sample type |
SRA |
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| Source name |
Midgut
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| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: Midgut
genotype: dC53EY22749
Sex: female
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| Treatment protocol |
size selection of RNAs less than 200 nucleotides in length using mirVANA RNA isolation kit AND demethylated using rtStar™ tRF&tiRNA Pretreatment Kit
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| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
Glasgow Polyomics
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| Library strategy |
ncRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
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| Data processing |
Read quality assessed with FastQC, and trimming has been done using cutadapt
Aligned to D. melanogaster genome and counted with Salmon
Analysed with DESeq2
Supplementary files format and content: containing read counts for genes analysed in corresponding samples
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| Submission date |
May 17, 2023 |
| Last update date |
Aug 26, 2024 |
| Contact name |
Nazif Alic |
| Organization name |
UCL
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| Street address |
Gower St
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| City |
London |
| ZIP/Postal code |
WC1E 6BT |
| Country |
United Kingdom |
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| Platform ID |
GPL19132 |
| Series (2) |
| GSE232719 |
Pol III transcribed genes, RNA-Seq, female fruit fly, midgut |
| GSE232724 |
RNA polymerase III in ageing, in C.elegans, fruit flies and mice |
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| Relations |
| BioSample |
SAMN35143950 |
| SRA |
SRX20403667 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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