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| Status |
Public on Jul 22, 2024 |
| Title |
ChIP-Seq Vernalized_leaf_H3K36me3_rep2 |
| Sample type |
SRA |
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| Source name |
Vernalized leaf
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| Organism |
Triticum aestivum |
| Characteristics |
tissue: Vernalized leaf
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP-seq was performed with antibodies for H3K27me3 (Abcam, ab6002), H3K4me3 (millipore, 07-473), H3K27ac (abclonal, A7253) and H3K36me3 (Abcam, ab9050). Libraries were constructed using Kit (TransGen Biotech, KP201-02), sequenced by Annoroad Gene Technology (Beijing, China).
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
We used fastp v0.21.0 for data quality control and Bowtie v2.3.5.1 to align reads to wheat reference genome (bowtie2 -X 1000 --very-sensitive).
We used fastp v0.21.0 for data quality control and Bowtie v2.3.5.1 to align reads to wheat reference genome (bowtie2 -X 1000 --very-sensitive).
Assembly: ChineseSpring v1.1
Supplementary files format and content: bigWig (except for Input sample).
Supplementary files format and content: Leaf.E1-9.segments.txt: Tab-delimited text file includes chromatin states of leaf.
Supplementary files format and content: Vernalized_leaf.E1-9.segments.txt: Tab-delimited text file includes chromatin states of vernalization leaf.
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| Submission date |
May 12, 2023 |
| Last update date |
Jul 22, 2024 |
| Contact name |
Zefu Lu |
| E-mail(s) |
[email protected]
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| Organization name |
Institute of Crop Sciences
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| Street address |
No.12 Zhongguancun South St.,Haidian District, Beijing, P.R.China
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| City |
Beijing |
| State/province |
Haidian |
| ZIP/Postal code |
100081 |
| Country |
China |
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| Platform ID |
GPL25409 |
| Series (2) |
| GSE232426 |
Identifications of vernalization regulatory elements by epigenomic approaches in winter wheat (ChIP-Seq) |
| GSE232430 |
Identifications of vernalization regulatory elements by epigenomic approaches in winter wheat |
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| Relations |
| BioSample |
SAMN35064001 |
| SRA |
SRX20313573 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM7333195_Vernalized_leaf_H3K36me3_rep2.bw |
191.5 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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