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Status
Public on Apr 03, 2012
Title
CD19+CD10+ cells2, miRNA
Sample type
RNA
Source name
Non-tumoral control
Organism
Homo sapiens Characteristics
tissue: CD19+CD10+ cells
Extracted molecule
total RNA
Extraction protocol
Total RNA was extracted by Trizol reagent (Invitrogen, Carlsbad, CA, USA).
Label
Cy3
Label protocol
100ng of total RNA were dephosphorylated, denaturalizated, treated with ligase T4 and labelled with Cy3 for 2 hours at 16ºC.
Hybridization protocol
Hybridization settings: 20 hours, at 55ºC, at 20rpm. Blocking agent, hybridization and washing buffers was performed according to Agilent's recommendations.
Scan protocol
Scan resolution 5um on an Agilent scanner.
Description
US22502553_251643612245_S01_miRNA-v1_10_Apr08_1_2_CD19+CD10+CELLS
Data processing
Limma package was used to import the data. After between-array median normalization by the R program, significant analysis of microarray (SAM) Excel macro was used.
Submission date
May 23, 2011
Last update date
Apr 03, 2012
Contact name
Lorena Di Lisio
E-mail(s)
[email protected] Organization name
CNIO
Street address
C/ Melchor Fernández Almagro, 3
City
Madrid
ZIP/Postal code
28029
Country
Spain
Platform ID
GPL8617 Series (2)
GSE23026 Combinatorial effects of microRNA to suppress the myc oncogenic pathway
GSE29491 Combinatorial effects of microRNA to suppress the myc oncogenic pathway (miRNA data)
Data table header descriptions ID_REF VALUE Normalized signal intensity
Data table ID_REF VALUE (-)3xSLv1
-1.926178656
A_25_P00010003
1.095833307
A_25_P00010004
-1.237070315
A_25_P00010005
8.618852332
A_25_P00010006
6.205305929
A_25_P00010007
2.688713803
A_25_P00010008
2.191415232
A_25_P00010009
1.905630266
A_25_P00010010
-1.935465362
A_25_P00010011
-2.054750805
A_25_P00010012
-3.689967108
A_25_P00010013
-3.80829614
A_25_P00010014
-1.924014648
A_25_P00010015
-1.60198709
A_25_P00010016
-1.986793545
A_25_P00010017
-1.688624025
A_25_P00010018
-5.600200997
A_25_P00010019
-0.80355112
A_25_P00010020
-1.029206198
A_25_P00010021
-4.665278443
1555 40 Kbytes .Supplementary file Size Download File type/resource GSM729702.txt.gz
607.6 Kb
(ftp) (http) TXT
Processed data included within Sample table
