|
| Status |
Public on Mar 06, 2012 |
| Title |
MDSC_BM_LALKO_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
cells (MDSC) isolated from LALKO Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD11b +ve Gr-1 +ve cells
tissue: bone marrow
age: 5 months
strain: 10 generation back-cross on FVB/N
genotype/variation: LALKO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the purified MDSC isolated from the Bone Marrow using the Qiagen total RNA purification kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA was prepared using the WT Terminal labelling kit according to standard affymetrix protocol. The Ovation Pico WTA System (Nugen) is used to prepare target cDNA. Finally the Encore Biotin Module (NuGEN) chemically fragments and labels the target sense cDNA
|
| |
|
| Hybridization protocol |
Biotinylated sense strand fragments were hybridized to Affymetrix Mouse Gene 1.0 ST GeneChips using the Hybridisation Control and Hybridisation Wash and Stain kits at 45° C for 18 hrs. Affymetrix GeneChip Scanner 3000 7G was used to scan the stained array
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000 7G was used to scan the stained array
|
| Description |
Gene profile of Myeloid derived Suppresive Cells from the Bone Marrow of Lysosomal Acid Lipase Knock-out Mice
|
| Data processing |
The data were analysed with GeneChip Operating Software 1.1(GCOS 1.1) using Affymetrix default analysis settings and all global scaling to the same target intensity value
|
| |
|
| Submission date |
May 19, 2011 |
| Last update date |
Mar 06, 2012 |
| Contact name |
Hong Du |
| E-mail(s) |
[email protected]
|
| Phone |
317-274-6535
|
| Fax |
317-278-2018
|
| Organization name |
Indiana University School of Medicine
|
| Department |
Pathology and Laboratory Medicine
|
| Street address |
635 Barnhill Drive
|
| City |
Indianapolis |
| State/province |
OH |
| ZIP/Postal code |
46202 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29401 |
Gene profile of Myeloid derived Suppressive Cells from the Bone Marrow of Lysosomal Acid Lipase Knock-out Mice |
|
