|
| Status |
Public on May 18, 2011 |
| Title |
mutant triceps, biological rep3 |
| Sample type |
RNA |
| |
|
| Source name |
triceps, mutant mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Nf1Prx1
tissue: triceps muscle
age: 3 months
|
| Treatment protocol |
Triceps muscle was isolated and directly homogenized in TriFast solution.
|
| Growth protocol |
3-month-old mice fed with standard chow.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the triceps muscles using peqGOLD TriFast (PeqLab Biotechnologie GmbH) according to the supplied protocol. The cRNA synthesis and microarray hybridizaton was done according to standard protocols applied in the Laboratory of Functional Genome Research Charité - Core Facility. A total of 8 high-density oligonucleotide mouse GENE 1.0 ST arrays (Affymetrix) were used in this study. Samples from each animal (4 Nf1, and 4 wild-type) were applied individually to each array.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on MouseGene1.0ST array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix).
|
| Description |
Gene expression data from 3-month-old triceps muscle.
|
| Data processing |
Data were processed using RMA (Affymetrix Expression Console software).
|
| |
|
| Submission date |
May 17, 2011 |
| Last update date |
Aug 29, 2011 |
| Contact name |
Mateusz Kolanczyk |
| E-mail(s) |
[email protected]
|
| Organization name |
Max Planck Institute
|
| Street address |
Ihnestr 73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6246 |
| Series (1) |
| GSE29343 |
Neurofibromin (Nf1) is required for skeletal muscle development |
|
