|
| Status |
Public on Jun 26, 2013 |
| Title |
032654 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
Amela-491
|
| Organism |
Mus musculus |
| Characteristics |
strain: TiRP Ink4a/ArfF/F B10.D2
sample: Amela-491
tissue: nonpigmented amelanotic melanoma
rna_number: 3
extract_amount: 5.0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Other
|
| Label |
Cy3
|
| Label protocol |
Ambion
|
| |
|
| Channel 2 |
| Source name |
Amela cell line 486
|
| Organism |
Mus musculus |
| Characteristics |
strain: TiRP Ink4a/ArfF/F B10.D2
sample: Amela cell line 486
cell type: nonpigmented amelanotic melanoma cells
cell line: Amela 486
rna_number: 3
extract_amount: 5.0
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Other
|
| Label |
Cy5
|
| Label protocol |
Ambion
|
| |
|
| |
| Hybridization protocol |
Agilent : 750.0 ng at 62 degree_C during 16 hours
|
| Scan protocol |
Scanned on a GenePix 4000B fluorescent scanner. Image intensity data were extracted with GenePix Pro 6.0 analysis software.
|
| Description |
Amela-491 vs. Amela cell line 486
Date:2007/04/04
|
| Data processing |
Interslide normalization: quantile, Intraslide normalization: printtiploess, Background normalization: Kooperberg, Software used: rscript. VALUE data obtained from log of processed Red signal/processed Green signal.
|
| |
|
| Submission date |
May 16, 2011 |
| Last update date |
Jun 26, 2013 |
| Contact name |
Kevin Lebrigand |
| Organization name |
IPMC/CNRS
|
| Lab |
Functional Genomics Platform of Nice-Sophia-Antipolis, France.
|
| Street address |
660 route des lucioles
|
| City |
Valbonne - Sophia-Antipolis |
| ZIP/Postal code |
06560 |
| Country |
France |
| |
|
| Platform ID |
GPL1476 |
| Series (1) |
| GSE29304 |
Gene expression profile analysis of inducible melanotic and amelanotic melanoma in mice |
|
