|
| Status |
Public on Oct 30, 2011 |
| Title |
6hrs light replica 2 |
| Sample type |
RNA |
| |
|
| Source name |
retinas collected at 1pm from rats kept all night in dark and then exposed to 3 hours of 1000 lux light
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: retina
treatment: 1pm from rats kept all night in dark and then exposed to 3 hours of 1000 lux light
strain: Long-Evans
|
| Treatment protocol |
3 or 6 hours of light exposure (1000 Lux).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA from retinas was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Data processing |
R/Bioconductor
|
| |
|
| Submission date |
May 14, 2011 |
| Last update date |
Oct 30, 2011 |
| Contact name |
Diana Eva Bedolla Orozco |
| E-mail(s) |
[email protected], [email protected]
|
| Phone |
+39-040-3787769
|
| Fax |
+39-040-3787702
|
| Organization name |
International School for Advanced Studies - SISSA
|
| Department |
Neurobiology
|
| Lab |
Lab. Torre
|
| Street address |
Via Bonomea 265
|
| City |
Trieste |
| ZIP/Postal code |
34136 |
| Country |
Italy |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE29299 |
Differential gene expression of adult rat retinas exposed to light. |
|
