|
| Status |
Public on Aug 31, 2005 |
| Title |
Fetal Kidney_day_22_treated_mother_rep10 |
| Sample type |
RNA |
| |
|
| Source name |
Fetal Kidney_day_22_treated_mother
|
| Organism |
Rattus norvegicus |
| Characteristics |
Wistar rat 22 day of gestation
|
| Biomaterial provider |
Ilana Ariel , Michael Bursztyn
|
| Treatment protocol |
Hyperinsulinemia was produced in pregnant female Wistar rats by subcutaneous implantation of a 2mm segment of a sustained release insulin implant (linplant, Linshin Canada Inc., Canada) a week before and again a week after mating. Female Wistar rats (weight, 200–280 g) were fed on a normal diet and allowed to drink tap water ad libitum. Blood pressure was measured weekly by the tail-cuff method. The experiment conducted as described in our previous investigations (Bursztyn at al., 1993; Podjarny et al., 1998; Bursztyn et al, 2003).
|
| Growth protocol |
Mother kept on a regular diet.4 in a cage.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the frozen tissue, using the RNA STAT-60TM Total RNA/mRNA Isolation Reagent (Tel-Test Inc., Friendswood, TX), according to the manufacturer’s instructions
|
| Label |
According to Affymetrix protocol
|
| Label protocol |
According to Affymetrix protocol
|
| |
|
| Hybridization protocol |
According to Affymetrix protocol
|
| Scan protocol |
According to Affymetrix protocol
|
| Description |
standard technique for comparisons of 5 normal samples to 5 samples of grows restricted fetuses
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Aug 29, 2005 |
| Last update date |
Aug 30, 2005 |
| Contact name |
Yoav M Smith |
| E-mail(s) |
[email protected]
|
| Phone |
02-6758495
|
| Fax |
02-6784010
|
| Organization name |
Hebrew University
|
| Department |
Medical School
|
| Lab |
Genomic Data Analysis
|
| Street address |
Hadassah Ein-Karem
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platform ID |
GPL341 |
| Series (1) |
| GSE3215 |
Gene Expression for fetal kidneys of hyper insulinemia induced intrauterine growth restriction rat model |
|
