|
| Status |
Public on May 10, 2011 |
| Title |
THRA aI mutant + vehicle replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
HepG2 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Hep G2
genotype: HCC-THRA mutant al
treatment: with vehicle for 6h
|
| Treatment protocol |
The HepG2 transformant pools expressing ectopic wildtype THRA, THRB, an HCC-TR mutant (αΙ or βN), a RCCC-TR mutant (6α or 15β), or the empty plasmid control were treated with 100 nM T3 or with ethanol carrier alone for 6h in DMEM containing 10% hormone-stripped fetal bovine serum.
|
| Growth protocol |
HepG2 cells were maintained at 37°C in Dulbecco's Modified Eagle's Medium (DME) supplemented with 10% fetal bovine serum using bicarbonate buffer and a 5% CO2 atmosphere.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The cells were harvested and the RNA was isolated using an RNeasy kit (Qiagen, Valencia CA).
|
| Label |
biotin
|
| Label protocol |
The purified RNA was provided to the University of California, Davis, Cancer Center Gene Expression Resource for subsequent cDNA probe generation and hybridization to Affymetrix GeneChip Human Gene 1.0 ST microarrays (Affymetrix Incorporated, Santa Clara CA).
|
| |
|
| Hybridization protocol |
The purified RNA was provided to the University of California, Davis, Cancer Center Gene Expression Resource for subsequent cDNA probe generation and hybridization to Affymetrix GeneChip Human Gene 1.0 ST microarrays (Affymetrix Incorporated, Santa Clara CA).
|
| Scan protocol |
The purified RNA was provided to the University of California, Davis, Cancer Center Gene Expression Resource for subsequent cDNA probe generation and hybridization to Affymetrix GeneChip Human Gene 1.0 ST microarrays (Affymetrix Incorporated, Santa Clara CA).
|
| Description |
HepG2 cells stably expressing HCC-THRA mutant al treated with vehicle for 6h.
|
| Data processing |
The raw microarray data was normalized by the Robust Multichip Array (RMA) method using R software.
|
| |
|
| Submission date |
May 09, 2011 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Scott Andrew Ochsner |
| E-mail(s) |
[email protected]
|
| Phone |
713-798-6227
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Cellular Biology
|
| Lab |
SPP: Signaling Pathways Project
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (1) |
| GSE29159 |
Mutant thyroid hormone receptors (TRs) isolated from distinct cancer types display distinct target gene specificities: a unique regulatory repertoire associated with renal clear cell carcinomas. |
|
| Relations |
| Reanalyzed by |
GSE86357 |
