|
| Status |
Public on May 12, 2023 |
| Title |
Sample 7_controlDiet |
| Sample type |
SRA |
| |
|
| Source name |
brain
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: brain
genotype: Canton-S
treatment: reference diet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol/Chloroform extraction of 10 pooled brains per sample.
RNA libraries for RNA-seq were prepared using NEBNext Single Cell/Low Input RNA Kit for Illumina following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
CLC Genomics Workbench v 11.0.1
Sequence reads were trimmed for adaptor sequence/low-quality sequence using FastP
Trimmed sequence reads were aligned to the Drosophila melanogaster transcriptome using STAR (parameters: -runThreadN -runMode genome Generate)
Read counts were calculated by couting all the reads that overlapped any exon for each gene using featureCounts.
Assembly: ftp.flybase.net/genomes/Drosophila_melanogaster/ version: r6.27
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
|
| |
|
| Submission date |
Apr 10, 2023 |
| Last update date |
May 13, 2023 |
| Contact name |
Scott D Pletcher |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Michigan
|
| Street address |
109 Zina Pitcher Pl
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL19132 |
| Series (1) |
| GSE229336 |
Effects of hunger on neuronal histone modifications slow aging in Drosophila |
|
| Relations |
| SRA |
SRX19920161 |
| BioSample |
SAMN34132575 |
