|
| Status |
Public on Sep 21, 2012 |
| Title |
Germinal center B cell (day 40), rep1 |
| Sample type |
RNA |
| |
|
| Source name |
IgG1 germinal center B cells (day40)
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
gender: female
tissue: spleen
age: 8 to 10 weeks old when immunized with NP-CGG/alum
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using TRIZOL reagent.
|
| Label |
biotin
|
| Label protocol |
Ovation RNA Amplification System V2 and FL-Ovation cDNA Biotin Module V2
|
| |
|
| Hybridization protocol |
Affymetrix standard procedures
|
| Scan protocol |
Using a microarray scanner as manufacture's instruction (Affymetrix)
|
| Description |
Mice were immunized with NP-CG/alum. On day 40 postimmunization, splenocytes were negatively selected with MACS system using biotinylated Abs against IgM, IgD, CD5, Gr1, NK1.1, DX5, TER119, F4/80, CD3 and CD90, followed by sorted NIP-binding B220+IgG1+CD38dullPNA+ fraction as germinal center B cells.
|
| Data processing |
GCRMA
|
| |
|
| Submission date |
Apr 22, 2011 |
| Last update date |
Sep 21, 2012 |
| Contact name |
Kaji Tomohiro |
| E-mail(s) |
[email protected]
|
| Phone |
81-45-503-9105
|
| Fax |
81-45-503-7053
|
| Organization name |
RIKEN
|
| Department |
Research center for allergy and immunology
|
| Lab |
Laboratory for Immunological memory
|
| Street address |
1-7-22, Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE11961 |
Gene expressions of memory B cells and other type of B cells |
|
