|
| Status |
Public on Nov 01, 2023 |
| Title |
PBS 2 |
| Sample type |
SRA |
| |
|
| Source name |
PBS 21 days
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Skin
cell type: Skin homogenate
Sex: male
strain: C57 BL/6 J
treatment: PBS
|
| Treatment protocol |
Phosphate buffered saline (PBS), group and bleomycin group were injected subcutaneously with 0.1 mL PBS and (0.1 units) bleomycin (Sigma, B5507), Celastrol groups were injected celastrol (Sigma, C0869) intraperitoneally with either 1mg/kg or 2mg/kg every day for 21 days
|
| Growth protocol |
4 weeks old male C57BL/6 J mice were ordered from Jackson lab and the treatment was started from 6 weeks old.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted using the TriZol extraction method.
Total RNA samples were processed using the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (PN NR603) from Vazyme Biotech Co. Ltd.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Partek Flow v10.0.22
fastq files were aligned to mm10 using Star 2.7.3a and annotated with Ensembl Transcripts release 102
Features were excluded if maximum reads <= 15.0
Reads were normalized using SESeq2 (poscounts)
Fold changes and p-values were caluclated using DESeq2.
Assembly: mm10
Supplementary files format and content: tab-delimited text file including fold change and statistical significance value for each gene
|
| |
|
| Submission date |
Mar 01, 2023 |
| Last update date |
Nov 01, 2023 |
| Contact name |
Andrew Leask |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Saskatchewan
|
| Department |
College of Dentistry
|
| Lab |
Leask Lab
|
| Street address |
105 Wiggins
|
| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7N5E4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE226375 |
The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis [In vivo bulk RNA-seq] |
| GSE226376 |
The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis |
|
| Relations |
| BioSample |
SAMN33553430 |
| SRA |
SRX19537951 |
