|
| Status |
Public on Nov 01, 2023 |
| Title |
TGFBneg_Celastrol_500nM |
| Sample type |
SRA |
| |
|
| Source name |
Celastrol 6h
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Skin
cell line: CCD-1077Sk
cell type: Fibroblasts
condition: Normal Skin
treatment: BSA+Citric Acid+Celastrol
|
| Treatment protocol |
Cells were treated with either DMSO or TGFB1 with or without Celastrol for 6h
|
| Growth protocol |
CCD-1077Sk cells (>P8) was cultured in high glucose DMEM+10%FBS+1%anti-anti until 70% confluency before treatment
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The RNA was extracted using the TriZol extraction method.
Total RNA samples were processed using the VAHTS Total RNA-seq (H/M/R) Library Prep Kit for Illumina (PN NR603) from Vazyme Biotech Co. Ltd.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Partek Flow v10.0.22
fastq files were aligned to hg38 using Star 2.7.3a and annotated with Ensembl Transcripts release 100
Features were excluded if maximum reads <=11.0
Reads were normalized using SESeq2 (median ratio)
Fold changes and p-values were caluclated using DESeq2.
Assembly: hg38
Supplementary files format and content: tab-delimited text file including fold change and statistical significance value for each gene
|
| |
|
| Submission date |
Mar 01, 2023 |
| Last update date |
Nov 01, 2023 |
| Contact name |
Andrew Leask |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Saskatchewan
|
| Department |
College of Dentistry
|
| Lab |
Leask Lab
|
| Street address |
105 Wiggins
|
| City |
Saskatoon |
| State/province |
Saskatchewan |
| ZIP/Postal code |
S7N5E4 |
| Country |
Canada |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE226374 |
The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis [in vitro bulk RNA-seq] |
| GSE226376 |
The Triptryium wilfordii derivative celastrol has anti-fibrotic effects in systemic sclerosis |
|
| Relations |
| BioSample |
SAMN33553498 |
| SRA |
SRX19537703 |
