|
| Status |
Public on Apr 21, 2011 |
| Title |
Tet1_shRNA2863 |
| Sample type |
RNA |
| |
|
| Source name |
mouse embryonic stem cells, Tet1 shRNA2863
|
| Organism |
Mus musculus |
| Characteristics |
strain: E14
cell type: mouse embryonic stem (MES) cells
treatment: Tet1 shRNA2863
|
| Treatment protocol |
E14 ES cells were infected with Scr shRNA, Tet1 shRNA2863 or Tet1 shRNA3387 containing lentivirus and harvested after 4-days selection with puromycin.
|
| Growth protocol |
E14 ES cells were feeder-free cultured.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol. At the final step of purification, RNA was loaded onto an RNeasy column (Qiagen) and treated with RNAse-free DNAse I.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Affymetrix One Cycle kit according to the standard Affymetrix protocol at Expression Analysis, Inc. (http://www.expressionanalysis.com)
|
| |
|
| Hybridization protocol |
Hybridization was performed according to the manufacturer's instructions at Expression Analysis, Inc. (http://www.expressionanalysis.com)
|
| Scan protocol |
Scanning was performed at Expression Analysis, Inc. (http://www.expressionanalysis.com)
|
| Description |
Gene expression data from E14 ES cells treated with Tet1 shRNA2863 and selected with puromycin.
|
| Data processing |
The data were analyzed with the MATLAB (R2009b) Bioinformatics toolbox affygcrma function with default analysis settings.
|
| |
|
| Submission date |
Apr 11, 2011 |
| Last update date |
Apr 21, 2011 |
| Contact name |
Feizhen Wu |
| E-mail(s) |
[email protected]
|
| Phone |
86-21-54237821
|
| Organization name |
Fudan Univ, Shanghai, China
|
| Department |
Institutes of Biomedical Sciences
|
| Lab |
Epigenetics lab
|
| Street address |
Dongan Road 131, Rm 511 Mingdao Building
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE28500 |
Genome-wide Regulation of 5hmC, 5mC and Gene Expression by Tet1 Hydroxylase in Mouse Embryonic Stem Cells |
| GSE28530 |
Genome-wide Regulation of 5hmC, 5mC and Gene Expression by Tet1 Hydroxylase in Mouse Embryonic Stem Cells (expression data) |
|
