|
| Status |
Public on Dec 04, 2013 |
| Title |
EVCT, control, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
EVCT cultured 48h
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: extravillous cytotrophoblasts
|
| Treatment protocol |
EVCT pellets were then snap-frozen and stored at -80°C until RNA extraction.
|
| Growth protocol |
EVCT were isolated by trypsin and purified on percoll gradient, then plated onto matrigel coated Petri dishes in DMEM/F12 10% FBS, cultured for 24h and then with or without rosiglitazone treatment for an additional 24 h period.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA were extracted from EVCT by Trizol (Invitrogen) followed by purification using RNeasy Mini kit (Quiagen). Integrity and purity of RNA were checked on Bioanalyzer 2100 with RNA 6000 LabChip kit (Agilent Biotechnologies)
|
| |
|
| Hybridization protocol |
20 µg of fragmented cRNA were hybridized for 16 hr at 45°C on Affymetrix GeneChip Human Genome U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneArray Scanner 3000. Expression values were generated with Microarray Suite v5.0 (MAS5, Affymetrix)
|
| Description |
Gene expression data from EVCT control
|
| Data processing |
MAS 5.0
|
| |
|
| Submission date |
Apr 06, 2011 |
| Last update date |
Dec 05, 2013 |
| Contact name |
Severine Aude Degrelle |
| E-mail(s) |
[email protected]
|
| Organization name |
INSERM UMRS-1139
|
| Street address |
4 avenue de l'Observatoire
|
| City |
PARIS |
| ZIP/Postal code |
75006 |
| Country |
France |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
|
