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Status
Public on Sep 20, 2005
Title
Melanocytes replicate 1
Sample type
RNA
Source name
Skin
Organism
Mus musculus Characteristics
FACSorted skin cells, melanocytes
Extracted molecule
total RNA
Extraction protocol
4 day old backskins were taken and Mx, ORS, DF, DP and Mc were FACSorted from Lef1-RFP/K14-H2BGFP mice. Total RNAs were prepared using the MicroPrep Kit (Stratagene)
Label
biotin
Label protocol
Approximately 1 µg of total RNA was processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Hair follicle melanocytes
Data processing
Affymetrix Microarray Suite version 5.0, scaled with the Affymetrix mask file to TGT=500
Submission date
Aug 16, 2005
Last update date
Sep 20, 2005
Contact name
Michael Rendl
E-mail(s)
[email protected] Organization name
Mount Sinai School of Medicine
Street address
1428 Madison Avenue
City
New York
State/province
NY
ZIP/Postal code
10029
Country
USA
Platform ID
GPL339 Series (1)
GSE3142 Molecular signatures in skin
Data table header descriptions ID_REF Affymetrix Identifier
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE 1428299_at
150.2
P
0.001953
1431226_a_at
4.3
A
0.5
1426662_at
466.3
P
0.000732
1419830_at
16.4
A
0.219482
1423763_x_at
4851.4
P
0.000244
1452718_at
869.5
P
0.000244
1417020_at
3
A
0.567627
1425279_at
69.8
P
0.010742
1435301_at
3.4
A
0.962402
1437330_at
35.5
A
0.219482
1452940_x_at
39.9
P
0.030273
1460190_at
51.4
A
0.398926
1416810_at
280.9
P
0.001953
1451337_at
356.1
P
0.00293
1455605_at
200.9
P
0.000244
1433952_at
70.9
A
0.067627
1416225_at
35.8
A
0.19458
1418354_at
36.1
A
0.129639
1448668_a_at
191.3
P
0.000244
1437155_a_at
175.5
P
0.00293
22690 609 Kbytes .Supplementary file Size Download File type/resource GSM70244.CEL.gz
3.6 Mb
(ftp) (http) CEL
GSM70244.EXP.gz
364 b
(ftp) (http) EXP
