|
| Status |
Public on Jun 01, 2011 |
| Title |
Experimental 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse placenta including decidua, diabetic
|
| Organism |
Mus musculus |
| Characteristics |
strain: FVB
age: E10.5
gender: female
tissue: placenta including decidua
phenotype: diabetic
|
| Treatment protocol |
Diabetes was induced in femal FVB mice by two intraperitoneal injections of 100mg/kg body weight Streptozotocin in 50mM sodium citrate buffer within a one week interval. Mice with a blood glucose level higher than 250mg/dL were considered diabetic and used for mating. The morning with the presence of a vaginal plug was counted as day 0.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Placentae were dissected at E10.5, weighted and stored in Trizol. Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled samples were generated using the Affymetrix Eukaryotic One-Cycle Target Labeling Assay.
|
| |
|
| Hybridization protocol |
GeneChip arrays were hybridized overnight in the Affymetrix hybridization oven at 42oC. Washing and scanning was performed using the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
| Description |
AE2 Diab
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0; Affymetrix GCOS software) using Affymetrix default analysis settings and global scaling as normalization method. Statistical evaluation was done with Cyber-T and GeneSpring.
|
| |
|
| Submission date |
Mar 30, 2011 |
| Last update date |
Jun 01, 2011 |
| Contact name |
Claudia Kappen |
| E-mail(s) |
[email protected]
|
| Phone |
225-763-2781
|
| Organization name |
Pennington Biomedical Research Center
|
| Department |
Developmental Biology
|
| Street address |
6400 Perkins Road
|
| City |
Baton Rouge |
| State/province |
LA |
| ZIP/Postal code |
70808 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE28277 |
Altered Gene Expression in Placentae from a Mouse Model of Diabetic Pregnancy |
|
