|
| Status |
Public on Mar 19, 2012 |
| Title |
Mitat |
| Sample type |
RNA |
| |
|
| Source name |
DCs from C57Bl/6 mice
|
| Organism |
Mus musculus |
| Characteristics |
treatment: treated with Mitat
cell type: Dendritic cells
strain: C57Bl/6
|
| Treatment protocol |
8 days old DCs were treated with appropriate stimuli for 24 hours at 37° and 5% CO2.
|
| Growth protocol |
Fresh bone marrow precursors were isolated from Tibia and Femur of 6-8 weeks old C57Bl/6 mice, depleted of CD90 and CD19 positive cells and cultivated for 8 days at 37° and 5% CO2 at 3e6 cells/well in 10 ml complete RPMI medium supplemented with 10% GM-CSF supernatant
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells were lysed in Trizol reagent and complete RNA was isolated according to manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (IVT-Express Kit, Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on a Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station_450
|
| Scan protocol |
GeneChips were scanned using the GeneArray Scanner 3000
|
| Description |
Gene expression data from DCs from C57Bl/6 mice, treated with Mitat
|
| Data processing |
The data were analyzed with Bioconductor packages (under R) and quantile-quantile as normalization method
|
| |
|
| Submission date |
Mar 29, 2011 |
| Last update date |
Mar 19, 2012 |
| Contact name |
Susanne Elma Kneitz |
| E-mail(s) |
[email protected]
|
| Phone |
+49-931-31 86526
|
| Organization name |
University of Wuerzburg
|
| Department |
Physiologigcal Chemistry
|
| Street address |
Biozentrum, Am Hubland
|
| City |
Wuerzburg |
| ZIP/Postal code |
97074 |
| Country |
Germany |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE28231 |
Dendritic cell maturation by proinflammatory TNF or pathogenic Trypanosoma brucei antigens instruct similar T helper-2 cell responses in murine models of autoimmunity and asthma |
|
