Data table header descriptions |
ID_REF |
|
VALUE |
log2 ratio of intensities (CH1I)/(CH2I) |
AVALUE |
average log2 total signal intensity |
GRID |
subarray identifier |
ROW |
row in the subarray |
COL |
column in the subarray |
CH1I |
uncorrected mean pixel intensity for channel 1 (Cy3) |
CH1B |
median background pixel intensity for channel 1 (Cy3) |
CH1AB |
mean background pixel intensity for channel 1 (Cy3) |
CH2I |
uncorrected mean pixel intensity for channel 2 (Cy5) |
CH2B |
median background pixel intensity for channel 2 (Cy5) |
CH2AB |
mean background pixel intensity for channel 2 (Cy5) |
SPIX |
number of pixels in a spot |
BGPIX |
uniform background in the two channels |
EDGE |
|
RAT2 |
|
MRAT |
median of (Ch2PI-CH2B)/(Ch1PI-CH1B) |
REGR |
slope estimate by simple linear regression |
CORR |
correlation between channel1 and channel 2 pixels within the spot |
LFRAT |
slope estimate by least-squares fit |
CH1GTB1 |
fraction of pixels in the spot greater than background (CH1B) |
CH2GTB1 |
fraction of pixels in the spot greater than background (CH2B) |
CH1GTB2 |
fraction of pixels in the spot greater than 1.5 times the background (CH1B) |
CH2GTB2 |
fraction of pixels in the spot greater than 1.5 times the background (CH2B) |
CH1EDGEA |
mean magnitude of the horizontal and vertical Sobel edge vectors contained within each spot (CH1) |
CH2EDGEA |
mean magnitude of the horizontal and vertical Sobel edge vectors contained within each spot (CH2) |
FLAG |
user defined spot flag |
CH1KSD |
value of the Komogorov-Smirnov statistic that assesses the likelihood that the spot pixel intensity distribution is drawn from the background distribution |
CH1KSP |
actual probability derived from CH1KSD |
CH2KSD |
value of the Komogorov-Smirnov statistic that assesses the likelihood that the spot pixel intensity distribution is drawn from the background distribution |
CH2KSP |
actual probability derived from CH2KSD |