|
| Status |
Public on Dec 20, 2023 |
| Title |
MSC, mutant#2, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
human iPS derived mesenchymal stem cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: human iPS derived mesenchymal stem cells
cell type: mesenchymal stem cells
genotype: Snord118 mutant heterozygote
|
| Treatment protocol |
MSCs were maintained in ɑ-MEM supplemented with 10% fetal bovine serum (FBS) and antibiotics in humidified atmosphere with 5% CO2 at 37℃
|
| Growth protocol |
Human iPS derived MSCs were induced from human iPS derived neural crest cells
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using TRIzol (ThermoFisher). 1 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using NEBNext® Ultra™ II RNA Library Prep Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequence reads were trimmed for adaptor sequence/low-quality sequence using trimmomatic v0.36
Trimmed sequence reads were mapped to GRCh38 with HISAT2 (v2.2.1) software
Read count extraction were performed using featureCounts (v2.0.1)
Assembly: hg38
Supplementary files format and content: tab-delimited text files include raw counts values for each Sample
|
| |
|
| Submission date |
Jan 24, 2023 |
| Last update date |
Dec 20, 2023 |
| Contact name |
Supawadee Jariyasakulroj |
| E-mail(s) |
[email protected]
|
| Phone |
2135955126
|
| Organization name |
University of Southern California
|
| Street address |
Alcazar street
|
| City |
Los Angeles |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE223614 |
Ribosome biogenesis controls suture mesenchymal stem cell fate via selective regulation of complement pathway in to prevent craniosynostosis [RNA-seq] |
| GSE223616 |
Ribosome biogenesis controls suture mesenchymal stem cell fate via selective regulation of complement pathway in to prevent craniosynostosis |
|
| Relations |
| BioSample |
SAMN32890561 |
| SRA |
SRX19152458 |
