|
| Status |
Public on Dec 08, 2011 |
| Title |
7H_seedling_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Barley chromosome addition line of common wheat
|
| Organism |
Triticum aestivum |
| Characteristics |
line: Hordeum vulgare cv. Betzes chromosome 7 addition line of Triticum aestivum cv. Chinese Spring
tissue: Leaf
|
| Growth protocol |
Seeds were germinated and grown on soil at 22 ºC under 16 hr light and 8 hr dark conditions for 2 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using an RNeasy Plant Mini kit (Qiagen). Total RNA quality was checked with an Agilent 2100 Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
A Cy3 labeled cRNA probe were prepared using a Low RNA Input Linear Amp Kit (Agilent Technologies), and microarray hybridization was performed following the recommended protocol.
|
| |
|
| Hybridization protocol |
Hybridization of the Cy3-labeled cRNA to the microarrays and washing were performed using a Gene Expression Hybridization kit and Gene Expression Wash Pack (Agilent Technologies) according to the manufacturer's instructions.
|
| Scan protocol |
Signal intensities were detected by Feature Extraction software (Agilent Technologies). Slides were scanned on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 1x44k array slides (Scan Area 61x21.6 mm, Scan resolution 5um, Dye channel is set to Green and Green PMT is XDR Hi 100% and XDR Li 10%).
|
| Description |
Hordeum vulgare cv. Betzes chromosome 7 addition line of Triticum aestivum cv. Chinese Spring, replicate 3
|
| Data processing |
The scanned images were analyzed with Feature Extraction Software 9.5 (Agilent Agilent Technologies) using default parameters(protocol GE1-v5_95) to obtain background subtracted and spatially detrended Processed Signal intensities. Features flagged in Feature Extraction as Feature Non-uniform outliers were excluded.
|
| |
|
| Submission date |
Mar 17, 2011 |
| Last update date |
Dec 08, 2011 |
| Contact name |
Jianwei Tang |
| E-mail(s) |
[email protected]
|
| Organization name |
Yokohama City University
|
| Department |
Kihara Institute for Biological Research
|
| Street address |
641-12 Maioka-cho, Totsuka-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
244-0813 |
| Country |
Japan |
| |
|
| Platform ID |
GPL9805 |
| Series (1) |
| GSE28023 |
Expression profiles of barley chromosome addition lines of common wheat |
|
