|
| Status |
Public on Dec 21, 2011 |
| Title |
Heart_Sham_2 |
| Sample type |
RNA |
| |
|
| Source name |
Swine left ventricle of Sham heart
|
| Organism |
Sus scrofa |
| Characteristics |
group: Sham
Sex: M
breed: Yorkshire x Landrace
|
| Treatment protocol |
Frozen myocardium was crushed in liquid nitrogen.
|
| Growth protocol |
2-3 months old swine
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol followed by Qiagen's Rneasy clean-up kit
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Labeled cRNA was hybridized to according to the manufacturer’s instructions
|
| Scan protocol |
standard Affymetrix protocol
|
| Data processing |
Data was analyzed with R. To prevent batch effect, data was first rma per group and then all arrays were quantile normalized.
|
| |
|
| Submission date |
Mar 15, 2011 |
| Last update date |
Dec 21, 2011 |
| Contact name |
Diederik Kuster |
| E-mail(s) |
[email protected]
|
| Organization name |
VU University Medical Center
|
| Street address |
van der Boechorststraat 7
|
| City |
Amsterdam |
| ZIP/Postal code |
1007 MB |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL3533 |
| Series (1) |
| GSE27962 |
Expression data of Sham and post-MI myocardium from swine |
|
