|
| Status |
Public on Mar 15, 2011 |
| Title |
p53null-mammary-tumor, TGFb-Het sham-irradiated host repeat 2 |
| Sample type |
RNA |
| |
|
| Source name |
Total RNA of p53null mammary tumor, TGFb-Het-Sham-irradiated host
|
| Organism |
Mus musculus |
| Characteristics |
strain (donor): BALB/c
tissue (donor): p53null mammary fragments
strain (host): BALB/c
genotype/variation (host): TGFb1 heterozygote
protocol (host): sham-irradiated
disease state (host): p53null mammary tumors
|
| Treatment protocol |
Host mice were either irradiated or not, three days before transplantation.
|
| Growth protocol |
p53null tumors developed from p53null mammary fragments that were orthotoically transplanted into the inguinal mammary fat pads of wild-type or TGFb1 heterozygous female host mice that had their endogenous epithelium removed at 3wks of age.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using Trizol Reaget (Invitrogen), according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
100 ng of total RNA were used to prepare cRNA following the Affymetrix 3’IVT Express Kit labeling protocol
|
| |
|
| Hybridization protocol |
12.5 micrograms of fragmented cRNA was hybridized for 16 hr to the Affymetrix MG 430 2.0 arrays using GeneChip Hybridization Oven 640 and Genechip® fluidics station FS 450, following standard Affymetrix protocols.
|
| Scan protocol |
Affymetrix GeneArray scanner 7G
|
| Description |
Het Sham-IR-host_19
From frozen mammary tumors
BALB/c wild-type RNA of tumors grown in BALB/c hosts
|
| Data processing |
Feature intensity was extracted by Affymetrix GeneChip Operating System as CEL files. The probe-level analysis of the CEL files was performed by the RMA algorithm using Agilent GeneSpring GX10 program. No further adjustments were made to the data in the table.
|
| |
|
| Submission date |
Mar 14, 2011 |
| Last update date |
Dec 19, 2012 |
| Contact name |
Jiri ZAVADIL |
| E-mail(s) |
[email protected]
|
| Phone |
+33648065733
|
| Organization name |
IARC WHO
|
| Street address |
150 COURS ALBERT THOMAS
|
| City |
LYON |
| State/province |
Other |
| ZIP/Postal code |
69008 |
| Country |
France |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE18216 |
Non-targeted effects of low dose ionizing radiation act via TGFβ to promote mammary carcinogenesis |
|
| Relations |
| Reanalyzed by |
GSM1049056 |
