|
| Status |
Public on Apr 08, 2011 |
| Title |
BIO586223 |
| Sample type |
RNA |
| |
|
| Source name |
siRNA COP1 scanned on 07/21/10
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCap
sirna transfection: siRNA COP1
|
| Treatment protocol |
siRNA oligonucleotides (Dharmacon) were transfected with Lipofectamine2000 and LipofectamineRNAiMax (Invitrogen) into LNCaP cells. Cell lysates were prepared 48h after siRNA transfection.
|
| Growth protocol |
LNCap cells were cultured overnight in serum-free medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total cellular RNA was prepared with a RNeasy Plus kit (Qiagen) and subject to DNAse I digestion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
HGU 133 plus 2.0 arrays were hybridized according to the Affymetrix protocol
|
| Scan protocol |
HGU 133 plus 2.0 arrays were scanned according to the Affymetrix protocol
|
| Data processing |
Affymetrix HGU 133 plus 2.0 intensity signal CEL files were processed using the RMA algorithm as implemented in the 'affy' package from Bioconductor.
|
| |
|
| Submission date |
Mar 11, 2011 |
| Last update date |
Apr 08, 2011 |
| Contact name |
Jinfeng Liu |
| E-mail(s) |
[email protected]
|
| Organization name |
Genentech Inc.
|
| Department |
Bioinformatics and Computational Biology
|
| Street address |
1 DNA Way, MS93
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (1) |
| GSE27914 |
Expression data from LNCap cell line treated with COP1 (RFWD2), ETV1, and JUN siRNAs |
|
