|
| Status |
Public on Feb 28, 2011 |
| Title |
YF050524MOT02 |
| Sample type |
RNA |
| |
|
| Source name |
mouse islet, 0h
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: islets
time after isolation: 0 hr
|
| Treatment protocol |
No treatment.
|
| Growth protocol |
Cultured in F12K medium with 10%FBS, 37°C, 5% CO2 incubator.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from islets with QIAGEN RNeasy Micro Kits.
|
| Label |
biotin
|
| Label protocol |
Target RNA was reverse transcribed into cDNA, and in-vitro transcription was performed to generate biotin-labeled cRNA for subsequent hybridization.
|
| |
|
| Hybridization protocol |
The labeled cRNA was hybridized on GeneChip Mouse Genome 430 2.0 Arrays. Hybridized target cRNA was stained with streptavidin phycoerythrin. See http://www.affymetrix.com/support/downloads/manuals/expression_analysis_technical_manual.pdf
|
| Scan protocol |
Arrays were scanned using a GeneArray Scanner at an excitation wavelength of 488nm. Light emissions at 570nm are proportional to the bound target at each oligonucleotides position on the GeneChip array. See http://www.affymetrix.com/support/downloads/manuals/expression_analysis_technical_manual.pdf
|
| Data processing |
RMA data were generated by McGill University Genome Quebec Innovation Centre through software FlexArray. Student t test was used to analyze the difference between groups.
|
| |
|
| Submission date |
Feb 27, 2011 |
| Last update date |
Feb 28, 2011 |
| Contact name |
bing han |
| E-mail(s) |
[email protected]
|
| Organization name |
university of montreal
|
| Department |
medicine
|
| Lab |
immunology
|
| Street address |
2099 alexandre deseve
|
| City |
montreal |
| State/province |
qc |
| ZIP/Postal code |
h2l2w5 |
| Country |
Canada |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE27547 |
Gene expression differences in mouse islets after isolation at different time points (0-48hr) |
|
