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Status
Public on Mar 05, 2006
Title
diabetic muscle replicate 1
Sample type
RNA
Source name
rear leg muscle
Organism
Rattus norvegicus Characteristics
Diabetic Male Wistar rat after 28 days of diabetes induced with streptozotocin
Extracted molecule
polyA RNA
Extraction protocol
Total RNA was obtained from approximately 100 mg of rear leg skeletal muscle stored in RNAlater® Qiagen (Valencia, CA) at -80oC using Qiagen RNeasy Maxi kits. Homogenates were made using a Brinkman/Kinetmatica Model PT-2100 polytron (Westbury, NY) for tissue dispersal. Total RNA samples were further processed for poly(A)+ mRNA isolation using Qiagen oligotex mRNA kit.
Label
biotin
Label protocol
Approximately 1 ug of mRNA processed to produce biotinylated cRNA targets.
Hybridization protocol
standard Affymetrix procedures
Scan protocol
standard Affymetrix procedures
Description
Data processing
Affymetrix Microarray Suite version 5.0
Submission date
Aug 05, 2005
Last update date
Dec 01, 2005
Contact name
Gail R Willsky
E-mail(s)
[email protected] Phone
716-829-2969
Fax
716 829-2725
Organization name
University at Buffalo
Department
Biochemistry
Lab
Willsky
Street address
140 Farber Hall
City
Buffalo
State/province
NY
ZIP/Postal code
14214
Country
USA
Platform ID
GPL85 Series (1)
GSE3068 normal and diabetic rat muscle treated and untreated with vanadyl sulfate
Data table header descriptions ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call
Data table ID_REF VALUE ABS_CALL DETECTION P-VALUE A01157cds_s_at
100.3
A
0.47938
A03913cds_s_at
414.9
A
0.089405
A04674cds_s_at
64.3
A
0.715253
A07543cds_s_at
279.9
A
0.47938
A09811cds_s_at
35.2
A
0.975289
A16585cds_s_at
72.2
A
0.302547
A17753cds_s_at
29.6
A
0.795978
A30543cds_s_at
46
A
0.795978
A44407cds_at
45.3
A
0.837065
AA108277_at
77
A
0.458816
AA108308_i_at
8.9
A
0.963203
AA108308_s_at
1086.4
A
0.189687
AA684537_at
2620
P
0.009985
AA684929_f_at
670.9
A
0.138765
AA684960_f_at
554.7
A
0.302547
AA684963_at
2384.2
P
0.001892
AA685112_at
2462.4
P
0.000266
AA685152_r_at
236.5
A
0.567627
AA685376_f_at
102.5
A
0.697453
AA685876_at
941
A
0.189687
8799 258 Kbytes .Supplementary data files not provided
