|
| Status |
Public on Dec 31, 2011 |
| Title |
Sick Mac 1 |
| Sample type |
RNA |
| |
|
| Source name |
Macrophages from nephritic, lupus nephritis kidneys
|
| Organism |
Mus musculus |
| Characteristics |
cell type: F4/80hi
strain: NZB/W
tissue: kidney
sample time: 2-6 weeks after proteinuria onset, but before the development of terminal renal failure
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using picopure RNA isolation kit (Arcturus Molecular Device Corporation, Sunnyvale, CA).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Refer to the Affymetrix gene expression manual
|
| Scan protocol |
GeneChip Scanner 3000 7G
|
| Description |
F4/80hi cell from NZB/W kidney (2-6 weeks after proteinuria onset, but before the development of terminal renal failure)
|
| Data processing |
The data were analyzed using GenePattern pipeline: RMA method and Mouse Entrez Gene custom CDF annotation version 10 were used for the normalization.
|
| |
|
| Submission date |
Feb 03, 2011 |
| Last update date |
Dec 31, 2011 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL7546 |
| Series (1) |
| GSE27045 |
Expression data from macrophages isolated from lupus NZB/W mouse kidneys prenephritic, nephritic or after complete remission. |
|
