|
| Status |
Public on Feb 11, 2011 |
| Title |
organism part name: V3_First Leaf and Sheath - rep2 |
| Sample type |
RNA |
| |
|
| Source name |
juvenile leaf,PO:0020104 leaf sheath (organism part name: V3_First Leaf and Sheath)
|
| Organism |
Zea mays |
| Characteristics |
germplasm name: Inbred line B73
organism part: PO:0006339 juvenile leaf,PO:0020104 leaf sheath
development stage: PO:0007065 LP.05 5 leaves visible,PO:0001053 3 leaf fully expanded
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL RNA extraction
|
| Label |
Cy3
|
| Label protocol |
NimbleGen One-Color DNA Labeling Kit
|
| |
|
| Hybridization protocol |
NimbleGen protocol for sample hybridization
|
| Scan protocol |
NimbleGen protocol for scanning one-color arrays
|
| Description |
V3_First Leaf and Sheath::First Leaf and Sheath::V3::Whole leaf and sheath was sampled.
|
| Data processing |
RMA normalization was performed using the rma() function in the oligo package of Bioconductor (www.bioconductor.org), modified to handle possible many-to-many relationships between probes and source sequences (summarization groups). The normalization scripts are available from the PLEXdb team by request.
|
| |
|
| Submission date |
Feb 01, 2011 |
| Last update date |
Feb 11, 2011 |
| Contact name |
PLEXdb Curator, Sudhansu Dash |
| Organization name |
Iowa State University
|
| Department |
Virtual Reality Application Center
|
| Lab |
PLEXdb
|
| Street address |
1023 Crop Genomes Informatics Lab (Iowa State University)
|
| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
| |
|
| Platform ID |
GPL12620 |
| Series (1) |
| GSE27004 |
Genome-wide gene expression atlas of maize inbred line B73 |
|
