NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM664125 Query DataSets for GSM664125
Status Public on Dec 21, 2011
Title ΔsigT x WT- rep2
Sample type RNA
 
Channel 1
Source name WT- sucrose
Organism Caulobacter vibrioides NA1000
Characteristics genotype/variation: wild type
stress: sucrose stress
Treatment protocol Exponentially growing cells were exposed to 150 mM sucrose for 30 min (mutant x parental cells) or harvested without any treatment (sigU overexpressing x double mutant cells)
Growth protocol Overnight cultures were diluted in fresh minimal medium supplemented with glucose to OD= 0.1
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy3
Label protocol standard Cy3-dCTP and Cy5-dCTP incorporations
 
Channel 2
Source name ΔsigT- sucrose
Organism Caulobacter vibrioides NA1000
Characteristics genotype/variation: sigT mutant
stress: sucrose stress
Treatment protocol Exponentially growing cells were exposed to 150 mM sucrose for 30 min (mutant x parental cells) or harvested without any treatment (sigU overexpressing x double mutant cells)
Growth protocol Overnight cultures were diluted in fresh minimal medium supplemented with glucose to OD= 0.1
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Trizol following manufacturer's instructions
Label Cy5
Label protocol standard Cy3-dCTP and Cy5-dCTP incorporations
 
 
Hybridization protocol Agilent protocol Agilent protocol Agilent protocol Agilent protocol
Scan protocol Agilent protocol Agilent protocol
Data processing Data were extracted and normalized using Agilent Feature Extraction Software 9.0. Agilent output files were then opened using Excel and the log10 values extracted with no additional processing or filtering
 
Submission date Jan 31, 2011
Last update date Dec 21, 2011
Contact name Rogerio Lourenco
E-mail(s) [email protected]
Organization name Universidade de Sao Paulo
Department Bioquimica
Street address Av. Prof. Lineu Prestes, 748
City Sao Paulo
State/province Sao Paulo
ZIP/Postal code 05508-900
Country Brazil
 
Platform ID GPL10469
Series (1)
GSE26977 PhyK, PhyR, σT and σU regulons

Data table header descriptions
ID_REF
VALUE normalized log10 (cy5/cy3) ratio

Data table
ID_REF VALUE
1 0.609
2 0.926
3 1.136
4 0.891
5 0.000
6 0.000
7 0.000
8 0.000
9 0.000
10 0.000
11 0.000
12 -0.926
13 0.217
14 0.321
15 0.072
16 0.014
17 0.199
18 -0.144
19 -0.155
20 -0.005

Total number of rows: 45206

Table truncated, full table size 540 Kbytes.




Supplementary file Size Download File type/resource
GSM664125.txt.gz 12.1 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap